Wilkins difficile toxin A . monoclonal antibody PCG - 4 on Clostridium Localization of two epitopes
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Characterization of toxins A and B of Clostridium difficile with monoclonal antibodies.
Two monoclonal antibodies (MAbs) were used to learn more about the structures of Clostridium difficile toxins A and B. One of the antibodies, the PCG-4 MAb, reacted specifically with toxin A. This MAb precipitated toxin A and neutralized the enterotoxic but not the cytotoxic activity of the toxin. The site to which the antibody bound was resistant to denaturation with sodium dodecyl sulfate; ho...
متن کاملEffects of Clostridium difficile toxins A and B in rabbit small and large intestine in vivo and on cultured cells in vitro.
Clostridium difficile is recognized as the major cause of antibiotic-associated colitis. C. difficile produces two toxins, A (enterotoxin) and B (cytotoxin), that are implicated in the pathogenesis of the colitis. We examined the dose responses, time course, and synergism of these two toxins in ligated rabbit intestinal loops and in tissue culture. In rabbit small intestinal loops, toxin A caus...
متن کاملPurification and characterization of Clostridium sordellii hemorrhagic toxin and cross-reactivity with Clostridium difficile toxin A (enterotoxin).
Hemorrhagic toxin (toxin HT) was purified from Clostridium sordellii culture filtrate. The purification steps included ultrafiltration through an XM-100 membrane filter and immunoaffinity chromatography, using a monoclonal antibody to toxin A of Clostridium difficile as the ligand. Toxin HT migrated as a major band with a molecular weight of 525,000 and a minor band at 450,000 on nondenaturing ...
متن کاملThe effect of clostridium difficile Toxins Aand B on ligated rabbit IIeal loop and cultured cell link BK
clostridium difficile has been recognized as the major cause of pseudomembranous colitis.this bacterium produces two toxins(an enterotoxin -cytotoxin and a potent cytotoxin called toxin A and toxin B erespectively).these toxins have implicated in pathogenesis of the disease.however,histopathological effects of their molecular mass less than 100KDa have been essayed.in the persent study,we exami...
متن کاملDouble Sandwich ELISA Modified Method for the Detection of Clostridium Botulinum Type E
Background & Objective: A very small amount of botulinum toxin can cause death and on the other hand, there is no cure for its poison other than antitoxin. Therefore, a diagnostic method that can detect very small amounts of botulinum toxin in a short time is very important. In this study, rapid and accurate detection of botulinum toxin type E has been performed with the double sandwich ELISA m...
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تاریخ انتشار 1992